September 7: Math problems

The problem with multipliers is that when you're off by a little... Your off by a magnitude. Such was my 96 well experiment that I played yesterday. I multipled by 2000ul instead of 200ul and washed out my cells. Solution: let excel do it for you. 

September 4: Vortex

September 1: it's 5am

And I'm doing my first round of drug treatments on my cells

August 28: Count twice treat once

First round of experiments. Fingers crossed that I:

#1: Plates the right number of cells

#2: Managed not to contaminate them

#3: Calculated the right concentration of drugs 

#4: Treated for the right length of time

So many ways for the Clinical Fellow Factor to duck my results 

August 26: FOILED

So today Leah and I spent an hour working out my first round of treatments. If we want to finish them at the same time they need to be staggered 12, 18

And 24 hours apart. They also need to be read when she's there to help use the machine then back track to count/plate the cells 48hrs before treatment. After all that work we settle on split today, treat

At noon, midnight and 0600 on Fri-Sat. Just to realzie oops it's 72 hours of treatments for the MTT and we didn't plate enough cells for the western. 

August 24: bug juice

After 2 contaminations in as many weeks, I found out we were supposed to be using pen-strep (1% penicillin-streptomycin) antibiotics in my media. Communication fail. 

August 17: gross contamination

Sacrificing my first cells in bleach